Journal: Journal of the Chinese Medical Association : JCMA

Article Title: TRIM72 mediates lung epithelial cell death upon hyperoxia exposure

doi: 10.1097/JCMA.0000000000000413

Figure Lengend Snippet: Immunohistochemistry results (A) and Western blotting results and quantitative data (B, C) for TRIM72 expression in 7-day-old and 14-day-old rats in the normoxia and O 2 -enriched groups. TRIM72 immunoreactivity was observed in endothelial cells (black arrow), superior surface of bronchial epithelial cells (red arrow), and alveolar type I (short black arrow) and II cells (short red arrow). The normoxia group showed faint immunoreactivity in bronchial epithelial cells (red arrow) and alveolar type I (short black arrow) and II cells (short red arrow). The rats in the O 2 -enriched group exhibited significantly higher TRIM72 expression than those in the normoxia group on postnatal days 7 and 14. * p < 0.05.

Article Snippet: RLE-6TN, a rat alveolar type II epithelial cell line, and A549, a human lung carcinoma epithelial cell line (ATCC, Manassas, VA, USA), were maintained in an F-12 medium in 75-cm 2 tissue culture flasks at 37.8°C in 5% CO 2 and 95% air.

Techniques: Immunohistochemistry, Western Blot, Expressing

Journal: Scientific reports

Article Title: Polyethylene glycol-phospholipid functionalized single-walled carbon nanotubes for enhanced siRNA systemic delivery.

doi: 10.1038/s41598-024-80646-1

Figure Lengend Snippet: Fig. 2. Significant gene silencing efficiency using SWNT-siRNA conjugates. (A) Graphical representation of SWNT-PL-PEG-S-S-siRNA. (B) Graphical representation of SWNT-PL-PEG-siRNA. (C, D and E) Evaluation of gene silencing effect by SWNT-PL-PEG-S-S-siGFP and SWNT-PL-PEG-siGFP conjugates in MCF-7, HEK-293T, H1299 and HeLa cells. Cells expressing stable green fluorescent protein (GFP) were treated with SWNT-PL-PEG-S-S-siGFP or SWNT-PL-PEG-siGFP conjugates at a concentration of 100 nM siGFP. The cell nuclei were stained with DAPI (blue). Microscopic observation was captured under 100x magnification. Bars represent mean ± SD of at least 3 independent experiments. * represents statistical significance as compared to vehicle controls (Student’s t-test, p < 0.01).

Article Snippet: Cell lines and cell culture The human breast cancer cell line MCF-7, human non-small cell lung carcinoma line H1299, and human cervical cancer cells HeLa were procured from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing, Concentration Assay, Staining

Journal: Scientific reports

Article Title: Polyethylene glycol-phospholipid functionalized single-walled carbon nanotubes for enhanced siRNA systemic delivery.

doi: 10.1038/s41598-024-80646-1

Figure Lengend Snippet: Fig. 3. Dual-silencing efficacy of siRNA-conjugated SWNT delivery system. Fluorescence microscopic images of co-expressed GFP and RFP in H1299 cells incubated with 100 nM of (A) SWNT-PL-PEG-S-S-siRNA or (B) SWNT-PL-PEG-siRNA conjugates targeting GFP (siGFP) or RFP (siRFP) or LUC (siLUC; negative control) alone, or both (dual targeting; siGFP + siRFP) for 72 h. The cell nuclei were stained with DAPI. The furthest right panel was the combined images. Microscopic observation was captured under 100x magnification.

Article Snippet: Cell lines and cell culture The human breast cancer cell line MCF-7, human non-small cell lung carcinoma line H1299, and human cervical cancer cells HeLa were procured from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Fluorescence, Incubation, Negative Control, Staining

Journal: Scientific reports

Article Title: Polyethylene glycol-phospholipid functionalized single-walled carbon nanotubes for enhanced siRNA systemic delivery.

doi: 10.1038/s41598-024-80646-1

Figure Lengend Snippet: Fig. 4. Energy dependent cellular internalization mechanisms of SWNT-siRNA conjugates independent of ABCB1. Graph shows the mean relative luminescent units (RLU) of (A and B) luciferase expressing H1299 cells co-treated with SWNT only (without siLUC), SWNT-PL-PEG-S-S-siLUC or SWNT-PL-PEG- siLUC with or without sub-lethal concentrations of various endocytosis inhibitors. NaN3, chlorpromazine, genistein and m-βCD are inhibitors for energy-dependent cellular uptake, clathrin-mediated endocytosis, caveolae-dependent endocytosis and macro-pinocytosis, respectively. Bars represent mean ± SD of at least 3 independent experiments. * represents statistical significance as compared to SWNT-siLUC (Student’s t-test, p < 0.01). (C) Western blot of ABCB1 and loading control GAPDH, cropped blots. Ectopic expression of ABCB1 (multiple drug resistance) protein in H1299-LUC cells was confirmed by the immunoblotting with increased expression of ABCB1 at 141 kDA, while GAPDH at 36 kDA served as the loading control. Full blots available in Supplementary Figure S2-S3. (D) Expression of ABCB1 did not affect the delivery of siRNA by the SWNT. Bars represent mean ± SD of at least 3 independent experiments. * represents statistical significance as compared to controls (Student’s t-test, p < 0.01).

Article Snippet: Cell lines and cell culture The human breast cancer cell line MCF-7, human non-small cell lung carcinoma line H1299, and human cervical cancer cells HeLa were procured from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Luciferase, Expressing, Western Blot, Control

Journal: Molecular cancer

Article Title: The reversion of DNA methylation-induced miRNA silence via biomimetic nanoparticles-mediated gene delivery for efficient lung adenocarcinoma therapy.

doi: 10.1186/s12943-022-01651-4

Figure Lengend Snippet: Fig. 1 miR-126-3p expression is silenced by hypermethylation of its promoter in lung cancer cells. A The miR-126-3p levels were measured by RT-qPCR in 67 pairs of lung adenocarcinoma tissues and adjacent non-tumor tissues. B RT-qPCR detected the miR-126-3p expression levels in BEAS-2B, A549, NCI-H1299, and H460 cells. C The overall survival of lung cancer patients with low or high miR-126-3p expression. D The relative expression levels of methylation of miR-126-3p were detected in tumor samples and adjacent lung samples by qRT-PCR. E MSP for methylation or demethylation of the miRNA-126-3p gene promoter in different LUAD and BEAS-2B cells. F The relative expression levels of miR-126-3p in different LUAD and BEAS-2B cells (treated with or without 5-Aza-dC for 24 h) were measured by RT-qPCR. *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: Human lung carcinoma cell lines H460, NCI-H1299, A549, and the normal human lung epithelial cell line BEAS-2B were obtained from ATCC (American Type Culture Collection, USA). hUV-MSCs and HUVECs were purchased from ScienCell Research Laboratories.

Techniques: Expressing, Quantitative RT-PCR, Methylation